Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Chodl mRNA is highly expressed in quiescent SCs (A) Chodl gene expression in various cell types in the mouse. Data were explored through the Tabula Muris data portal. (B) Relative expression of the Chodl gene in SCs, bladder cells, and a non-annotated cell population in (A). (C) UMAP-embedding of the scRNA-seq data on SCs isolated from non-injured muscles and injured muscles at 5 dpi in 2-month-old mice. (D) Violin plots show subcluster-specific gene expression ( Pax7 , Myog , Mki67 ) and enrichment of Chodl in the QSCs and ASCs. Colored by cluster identity. Abbreviations: QSC, quiescent SCs; SSC, self-renewal SCs; ASC, activated SCs; PSC, proliferating SCs; CSC, committed SCs; DSC, differentiating SCs. (E) qRT-PCR analysis of Chodl expression level in mouse QSC, ASC, and myoblast. n = 3 mice. (F) Immunoblot analysis showing CHODL expression in mouse myoblasts during myogenic differentiation. Relative band densitometry was calculated using ImageJ. (G) qRT-PCR analysis of Chodl in TA muscles post CTX injury in the mouse. n = 4 mice. Data are represented as mean ± SEM. The p values in E and G were analyzed using one-way ANOVA and Tukey post hoc test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: To gain insight into the expression pattern of Chodl across various tissues, we conducted a comprehensive analysis using the scRNA-seq data publicly available from Tabula Muris , Our analysis revealed that Chodl mRNA was mainly enriched in SCs, bladder cells, and a non-annotated cell population, while not detected in other cell types, including fibro-adipogenic progenitors (FAPs) ( A and 1B).

Techniques: Gene Expression, Expressing, Isolation, Muscles, Quantitative RT-PCR, Western Blot, Cell Characterization